Towards sustainable bioplastic production using the photoautotrophic bacterium Rhodopseudomonas palustris TIE-1

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Isolation and characterization of a genetically tractable photoautotrophic Fe(II)-oxidizing bacterium, Rhodopseudomonas palustris strain TIE-1.

We report the isolation and characterization of a phototrophic ferrous iron [Fe(II)]-oxidizing bacterium named TIE-1 that differs from other Fe(II)-oxidizing phototrophs in that it is genetically tractable. Under anaerobic conditions, TIE-1 grows photoautotrophically with Fe(II), H2, or thiosulfate as the electron donor and photoheterotrophically with a variety of organic carbon sources. TIE-1 ...

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Biohydrogen production from synthesis gas (syngas) was investigated using a local bacterium isolated from anaerobic sludge of dairy wastewater. The isolated strain Rhodopseudomonas palustris PT was able to convert syngas to hydrogen through water shift gas (WGS) reaction. The aim of present study was to determine the optimum concentration of ferric citrate which avoids the cell growth inhibitio...

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Metabolic Flexibility and Substrate Preference by the Fe(II)-Oxidizing Purple Non-Sulphur Bacterium Rhodopseudomonas palustris Strain TIE-1

It is unknown to which extent phototrophic Fe(II) oxidation takes place in the simultaneous presence of organic electron donors (e.g., acetate/lactate). Therefore, the photoferrotrophic strain Rhodopseudomonas palustris TIE-1 was inoculated with various combinations of Fe(II), acetate and lactate to understand metabolic substrate preference. When acetate was provided together with Fe(II), TIE-1...

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Biogeography of the purple nonsulfur bacterium Rhodopseudomonas palustris.

The biogeography of the purple nonsulfur bacterium Rhodopseudomonas palustris on a local scale was investigated. Thirty clones of phototrophic bacteria were isolated from each of five unevenly spaced sampling locations in freshwater marsh sediments along a linear 10-m transect, and a total of 150 clones were characterized by BOX-PCR genomic DNA fingerprinting. Cluster analysis of 150 genomic fi...

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The pio operon is essential for phototrophic Fe(II) oxidation in Rhodopseudomonas palustris TIE-1.

Phototrophic Fe(II)-oxidizing bacteria couple the oxidation of ferrous iron [Fe(II)] to reductive CO(2) fixation by using light energy, but until recently, little has been understood about the molecular basis for this process. Here we report the discovery, with Rhodopseudomonas palustris TIE-1 as a model organism, of a three-gene operon, designated the pio operon (for phototrophic iron oxidatio...

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ژورنال

عنوان ژورنال: Journal of Industrial Microbiology and Biotechnology

سال: 2019

ISSN: 1476-5535,1367-5435

DOI: 10.1007/s10295-019-02165-7